来自新石栖热袍菌的β-葡萄糖苷酶 Beta-Glucosidase from Thermotoga neopolitana

A thermostable β-glucosidase. The enzyme catalyses the transfer of D-glucose residues in disaccharides, alkyl glycosides or other aglycone-linked glycosides to water (hydrolysis) or to alcohols depending on reaction conditions.

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Enzyme activity: Bgl162 β-glucosidase catalyses deglycosylations and transglycosylation reactions with retaining mechanism. The enzyme can both catalyse hydrolysis of various glycosides and transglycosylations with alcohols.

Activity determination: Glycosidase activity was determined at 85°C in a 20 mM citrate phosphate buffer at pH 5.6 and incubated for 5 minutes with 2.92 mM final concentration of p-nitrophenyl-β-D-glucanopyranoside.

Unit definition: One unit (U) of enzyme activity is the amount that leads to the release of 1 μmol of p-nitrophenyl from p-nitrophenyl-β-D-glucanopyranoside (pNPG) per minute.

Synonyms: β-glucosidase, glycoside hydrolase, β-D-glucoside glucohydrolase

Protein family: Glycosyl hydrolase family 3 ( GH3 ) – CAZy database GH3 family

Enzyme classification: EC 3.2.1.6

Source: The thermophilic bacteria Thermotoga neopolitana

Protein sequence: NBCI protein entry

Structural information: The crystal structure of β-glucosidase from Thermotoga neopolitana has been determined to 2.05 Å resolution (Pozzo et al. 2010). – PDB entry 2X41