Phadebas®DLA2（检测低淀粉酶活性）

 Phadebas® DLA2 is a product for detection and semi-quantification of lower levels of α-Amylase specifically. Could be used e.g. in quality control for α-amylase residues in Food Production Industry and Research.

A DLA2 dish has 7 sample wells, one for negative control and 6 for samples or references. Liquid samples can be analyzed, independent of composition, coloring and opacity.

DLA2 is Ready to Use, Easy to Use, Easy to Evaluate and requires low work effort and equipment

Q&A Phadebas® DLA2

Q: Can I reuse the dish?

A: No, not even if there are non used wells.

Q:Why am I not getting any clear phase?

A: The amylase activity is below detection level.

– The sample does not contain any α-amylase.

– The α-amylase in the sample has been deactivated.

– No sample has been added.

Q: How should I dilute the concentrated α-amylase solution before use as a reference?

A; Dilute your α-amylase reference freshly with deionised water before application and

in general, they can be diluted 100 000- 1000 000 times.

Q: Can I run different samples on the same plate?

A: Yes, if your samples contain the same type of α-amylase and could be detected at the same temperature.

Q: Why do I get a clear zone in the negative control?

A: Your pipette, pipette tips, water etc. is contaminated with α-amylase.

Q: What should I do if a clear zone interact with other clear zones?

A: Dilute your sample with deionized water and run your sample ones more. Remember dilution factor.

Q: Can I analyse opaque and colored samples?

A: Yes, Sample must be in the liquid form, if not dilute with deionised water.

Q: Why do I not get any detection of my diluted reference sample?

A: It is important that your reference samples are freshly prepared prior to use, due to the fact that α- amylase can lose activity over time.

Q: Can I incubate the dish for more than 16 hours?

A: Yes, but there is a risk of dehydrating and cracking of the gel.

Q: Why do I get clear zones not connected to the wells?

A; The dish has been contaminated with α-amylase from undefined source.

The samples should be reanalysed.

Q; Could bacteria, yeast or mold grow on the dish?

A; No, there is a preservative added to the dish to avoid this.

Q; Is it possible to detect other amylases or enzymes than α-amylase?

A; No, this dish is specific for α-amylase detection.

Phadebas®DLA2是一种专门用于检测和半定量较低水平α-淀粉酶的产品。可用于食品生产工业和研究中α-淀粉酶残留的质量控制。

DLA2培养皿具有7个样品孔，一个用于阴性对照，6个用于样品或参考。液体样品可以独立于成分、颜色和不透明度进行分析。

DLA2即用即用，易于使用，易于评估，并且需要较少的工作量和设备

问答Phadebas®DLA2

Q： 我可以重复使用这道菜吗？

A： 不，即使有未使用过的井也不会。

Q： 为什么我没有得到任何明确的阶段？

A： 淀粉酶活性低于检测水平。

–样品不含任何α-淀粉酶。

–样品中的α-淀粉酶已失活。

–未添加任何样本。

Q： 在用作参考之前，我应该如何稀释浓缩的α-淀粉酶溶液？

A.使用前，用去离子水新鲜稀释α-淀粉酶对照品

一般来说，它们可以稀释10万到10万倍。

Q： 我可以在同一个盘子上运行不同的样品吗？

A： 是的，如果你的样品含有相同类型的α-淀粉酶，并且可以在相同的温度下检测到。

Q： 为什么我在阴性对照中有一个明确的区域？

A： 您的移液管、移液管尖端、水等被α-淀粉酶污染。

Q： 如果一个透明区域与其他透明区域相互作用，我该怎么办？

A： 用去离子水稀释您的样品，再运行一次样品。记住稀释系数。

Q： 我可以分析不透明和有色的样品吗？

A： 是的，如果没有用去离子水稀释，样品必须是液体形式。

Q： 为什么我的稀释参考样品没有得到任何检测？

A： 重要的是，您的参考样品在使用前是新鲜制备的，因为α-淀粉酶会随着时间的推移而失去活性。

Q： 我能把这道菜培养16个小时以上吗？

A： 是的，但凝胶有脱水和破裂的风险。

Q： 为什么我要得到没有连接到井的清晰区域？

A.这道菜被不明来源的α-淀粉酶污染了。

应重新分析样品。

Q细菌、酵母或霉菌会在盘子里生长吗？

A.不，为了避免这种情况，在菜里添加了防腐剂。

Q有可能检测到除α-淀粉酶以外的其他淀粉酶或酶吗？

A.不，这道是专门检测α-淀粉酶的。