商品描述
重组人 IL-7 蛋白
产品规格
纯度:>97%,通过还原条件下的 SDS-PAGE 和银染可见。
内毒素水平:LAL 法测定的每 1 μg 蛋白质 <0.01 EU。
活性:使用 PHA 激活的人外周血淋巴细胞 (PBL) 在细胞增殖试验中测量。横田,T. 等人。 (1986)过程。国家石油公司。学院派。科学。美国 83:5894。这种效果的 ED50 为 0.1-0.5 ng/mL。
重组人 IL-7 的比活性约为 4.4 x 105 IU/μg,根据人 IL-7 WHO 国际标准(NIBSC 代码:90/530)进行校准。具体活动仅供参考,不进行常规测试。
来源:大肠杆菌衍生的人 IL-7 蛋白
Asp26-His177,具有 N 端 Met
入藏号:P13232
N端序列分析:Met
预测分子量:17 kDa
配方:从 0.2 μm 过滤的 PBS 溶液冻干而成。 * 1 mg 包装尺寸 (01M) 作为 0.2 µm 过滤溶液在 PBS 中提供。
复溶:在无菌 PBS 中以 50 μg/mL 复溶 5 μg 小瓶。在无菌 PBS 中以 100 μg/mL 重构 10 μg 或更大的小瓶。
运输:产品在环境温度下运输。收到后,立即将其存放在以下推荐的温度下。
稳定性和储存:使用手动除霜冷冻机,避免重复冻融循环。
自收到之日起 12 个月,-20 至 -70 °C(按供应)。
1 个月,2 至 8 °C 在无菌条件下复溶后。
3 个月,-20 至 -70 °C 在无菌条件下复溶后。
Recombinant Human IL-7 Protein
Product Specifications
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
Activity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530). Specific activity is for reference purposes only and is not routinely tested.
Source: E. coli-derived human IL-7 protein
Asp26-His177, with an N-terminal Met
Accession #: P13232
N-terminal Sequence Analysis: Met
Predicted Molecular Mass :17 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution: Reconstitute 5 μg vials at 50 μg/mL in sterile PBS. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.