商品描述
磷酸化在许多细胞功能的调节中起着关键作用。常见的磷酸化检测方法包括昂贵且通常不可用的磷酸化抗体或不安全的放射性ATP标记。我们提供pIMAGO?C是一种新型、非抗体、高效且安全的产品,可无偏见地普遍检测任何磷蛋白。与抗体不同,pIMAGO能够独立于氨基酸序列选择性结合到磷酸化残基。该pIMAGO试剂盒能够使用化学发光(HRP/过氧化物酶)检测Western Blot格式(膜上)的磷酸蛋白。该试剂盒包括pIMAGO生物素试剂、抗生物素HRP(抗生物素过氧化物酶)检测试剂、阻断缓冲液、pIMAGO缓冲液、洗涤缓冲液和对照磷酸蛋白。不包括ECL基板。
Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling. We offer pIMAGO – a novel, non-antibody, highly effective and safe product for unbiased universal detection of any phosphoprotein. Unlike antibodies, pIMAGO is capable of selective binding to a phosphorylated residue independent of amino acid sequence. This pIMAGO kit enables the detection of phosphoproteins in Western Blot format (on-membrane) using chemiluminescence (HRP/peroxidase). The kit includes pIMAGO-biotin reagent, avidin-HRP (avidin-peroxidase) detection reagent, blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein. ECL substrate is not included.